PXD066546

PXD066546 is an original dataset announced via ProteomeXchange.

Title	Beyond Traditional SPAAC: Achieving Orthogonality and Rapid Kinetics with Fluoroalkyl Azides
Description	Selective modification of biological systems via click reactions is an important method to study their properties and function under native conditions. One of the most used method is the strain-promoted azide-alkyne cycloaddition (SPAAC) reaction between azides and strained cyclooctynes. In this work, we show, that fluorination of the azide reagents enhances their click reactivity and enables achieving of excellent selectivity when used in combination with simple alkyl azides. This work extends the available toolkit for efficient and selective modification of (bio)molecules under benign, metal-free condition.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:12:50.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frantisek Filandr
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-24 09:02:52	ID requested
⏵ 1	2026-05-24 16:12:51	announced

Tom, č, o M, Š, lachtov, á V, Vr, á, bel M, Li J, Filgas J, Slav, í, č, ek P, Bedn, á, rov, á L, Filandr F, Beier P, Beyond traditional strain-promoted azide-alkyne cycloadditions by achieving orthogonality and rapid kinetics with fluoroalkyl azides. Commun Chem, 9(1):(2026) [pubmed]

10.1038/s42004-026-01927-6;

submitter keyword: LC-MS, PTM, Top-Down, mAb

Milan Vrábel
contact affiliation	Institute of Organic Chemistry and Biochemistry of the CAS
contact email	milan.vrabel@uochb.cas.cz
lab head
Frantisek Filandr
contact affiliation	Institute of Organic Chemistry and Biochemistry of the CAS
contact email	filandr.frantisek@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD066546

PRIDE project URI

• PRIDE
  1. PXD066546
     1. Label: PRIDE project
     2. Name: Beyond Traditional SPAAC: Achieving Orthogonality and Rapid Kinetics with Fluoroalkyl Azides