PXD066535

PXD066535 is an original dataset announced via ProteomeXchange.

Title	RPN1 pulldown ID with PSMD5 overexpression from 'Integrated structural biology and in situ crosslinking reveals UBE3C, TXNL1, and PSMD5 at proteasome complexes'
Description	For PSMD5 over expression experiment, RPN1-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_08:49:37.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kitaik Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; mono N-acetylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-24 07:38:03	ID requested
⏵ 1	2026-07-16 08:49:37	announced

10.1016/J.MOLCEL.2026.06.032;

submitter keyword: Human, Proteasome

Francis J.O'Reilly
contact affiliation	Center for Structural Biology, Center for Cancer Research, National Cancer Institute (NCI), National Institutes of Health, Frederick, MD 21702-1201, USA
contact email	oreillyfj@nih.gov
lab head
Kitaik Lee
contact affiliation	Center of Excellence for Data Driven Discovery, Department of Structural Biology, St Jude Children’s Research Hospital, Memphis, Tennessee 38105, United States
contact email	kitaik.lee@stjude.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD066535

PRIDE project URI

• PRIDE
  1. PXD066535
     1. Label: PRIDE project
     2. Name: RPN1 pulldown ID with PSMD5 overexpression from 'Integrated structural biology and in situ crosslinking reveals UBE3C, TXNL1, and PSMD5 at proteasome complexes'