PXD066510

PXD066510 is an original dataset announced via ProteomeXchange.

Title	Sleep fragmentation exacerbates cardiac microvascular endothelial cell cuproptosis via promoting neutrophil extracellular trap formation in myocardial ischemia-reperfusion injury
Description	Objective: This study aimed to investigate the relationship between sleep disorders and cardiac microvascular injury in myocardial ischemia-reperfusion injury (MI/RI) mice. Design: Mice were placed in a sleep deprivation chamber. The sweep bar moved along the bottom of the cage every 2 min during the light cycle (ZT0-12) and was stationary during the dark cycle (ZT12-24) for 16 weeks. Then, a MI/RI model was prepared to evaluate cardiac microvascular injury and investigate its specific mechanism via proteomics analysis. Results: Here, we showed that 16 weeks of sleep fragmentation (SF) exacerbated cardiac microvascular injury in MI/RI mice. Mechanistically, we found that SF promotes sympathetic overactivity, increases the level of norepinephrine (EPI) in plasma, thereby promotes the chemotaxis of neutrophils (NEs) and formation of Neutrophil extracellular traps (NETs). Furthermore, our data indicated that NETs inhibit Atox1 expression and leads to impaired ATP7A related copper transport and copper overload in cardiac microvascular endothelial cells (CMECs). Copper overload further leads to exacerbated cuproptosis, and these effects can be rescued by excision of the sympathetic nerve, targeted administration of copper chelating agent or NETs inhibitor. Conclusions: This study demonstrated that SF exacerbated MI/RI by promoting copper overload in CMECs. Our study elucidated one of the molecular mechanisms by which sleep disorders aggravate cardiac microvascular injury and suggested possible tar gets for intervention.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:30:23.910.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongli Sun
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-24 00:54:02	ID requested
⏵ 1	2026-06-07 16:30:24	announced

Shi P, Sha Y, Guan X, Wu J, Yang J, Min D, Wang C, Cao Y, Wang X, Qiao Y, Sun H, Neuromodulation and Copper Chelation Reverse Sleep Fragmentation-Aggravated Myocardial Ischemia-Reperfusion Injury by Targeting NET-Induced Endothelial Cuproptosis. Research (Wash D C), 9():1266(2026) [pubmed]

10.34133/research.1266;

submitter keyword: Myocardial ischemia-reperfusion injury

Sleep fragmentation

Neutrophil extracellular traps

Cardiac microvascular endothelial cells

Cuproptosis

Hongli Sun
contact affiliation	Department of Pharmacology, Harbin Medical University, Heilongjiang 163319, China
contact email	shlhmu2018@163.com
lab head
Hongli Sun
contact affiliation	Harbin Medical Universiy
contact email	shlhmu2018@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066510
     1. Label: PRIDE project
     2. Name: Sleep fragmentation exacerbates cardiac microvascular endothelial cell cuproptosis via promoting neutrophil extracellular trap formation in myocardial ischemia-reperfusion injury