PXD066496

PXD066496 is an original dataset announced via ProteomeXchange.

Title	MCF7 treated with abemaciclib nuclear Rb IP LC-MSMS
Description	The retinoblastoma protein (Rb) is a tumour suppressor best known for repressing E2F transcription factors and halting cell cycle progression1. In hormone receptor-positive (HR+) breast cancer, CDK4/6 inhibitors activate Rb by preventing its phosphorylation, forming a key component of current endocrine therapy regimens2. How pharmacologically activated Rb remodels chromatin and influences transcriptional networks beyond cell cycle arrest remains poorly understood. We show that CDK4/6 inhibition induces widespread redistribution of hypo-phosphorylated Rb to both promoters and enhancers in cancer cells. While Rb predictably binds to cell cycle gene promoters to repress transcription, at other sites it unexpectedly promotes expression of oestrogen-responsive genes by integrating into oestrogen receptor (ER)-rich transcriptional hubs. In this dataset, we investigate chromatin modifiers that can interact with Rb in the nucleus with CDK4/6 inhibitor treatment in the ER+ breast cancer cell line MCF7.
HostingRepository	PRIDE
AnnounceDate	2026-06-23
AnnouncementXML	Submission_2026-06-22_21:40:56.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	April C. Watt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-23 20:51:04	ID requested
⏵ 1	2026-06-22 21:40:56	announced

Dataset with its publication pending

submitter keyword: Rb, LC-MSMS, CDK4/6i, IP,Breast cancer

Shom Goel
contact affiliation	Sir Peter MacCallum Department of Oncology, University of Melbourne, Australia; Peter MacCallum Cancer Centre, Melbourne, Australia
contact email	shom.goel@petermac.org
lab head
April C. Watt
contact affiliation	Sir Peter MacCallum Department of Oncology, University of Melbourne, Australia; Peter MacCallum Cancer Centre, Melbourne, Australia
contact email	april.watt@petermac.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD066496

PRIDE project URI

• PRIDE
  1. PXD066496
     1. Label: PRIDE project
     2. Name: MCF7 treated with abemaciclib nuclear Rb IP LC-MSMS