PXD066489

PXD066489 is an original dataset announced via ProteomeXchange.

Title	Phospho-proteomic analysis of IL-13 treated human airway epithelial cell line (A549)
Description	Allergic asthma is a chronic inflammatory airway disease driven by the cytokine interleukin-13 (IL-13). Although IL-13 signals through the canonical JAK1/TYK2/STAT6 pathway, our understanding of the totality of IL-13-induced signaling intermediates is incomplete. To address this, we performed an unbiased phospho-proteomic analysis of IL-13-stimulated A549 human airway epithelial cells. IL-13 stimulation led to differential phosphorylation at 145 unique serine/threonine residues across 97 proteins involved in diverse cellular processes. These processes included RNA splicing, cytoskeletal remodeling, GTPase activity, and focal adhesion complex formation. Network analysis identified SRC, a non-receptor tyrosine kinase, as a potential upstream regulator of IL-13-induced changes in phosphorylation. Kinetic molecular approaches confirmed that SRC is rapidly activated following IL-13 stimulation, prior to activation of the canonical IL-13 signaling intermediate STAT6 in both human and mouse lung fibroblasts. Pharmacological inhibition of SRC reduced IL-13–induced STAT6 phosphorylation and downstream gene expression in vitro. In vivo, SRC antagonism attenuated IL-13–induced airway hyperresponsiveness (AHR) without significantly affecting inflammatory cell infiltration or gene expression in bronchoalveolar lavage fluid. These findings identify SRC as a novel and selective mediator of IL-13–driven airway responses and suggest that targeting SRC may offer therapeutic benefit in allergic asthma.
HostingRepository	PRIDE
AnnounceDate	2025-12-05
AnnouncementXML	Submission_2025-12-04_19:37:03.116.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Archana Shankar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-23 14:14:23	ID requested
⏵ 1	2025-12-04 19:37:04	announced

Shankar A, McAlees JW, Wyder MA, Hargis JM, Ediga HH, Cannata A, Latayan J, Greis KD, Madala SK, Lewkowich IP, Phospho-Proteomic Analysis of Interleukin-13 Signaling in Airway Cells Reveals SRC Family Kinase Involvement in Interleukin-13-Induced Inflammatory Responses. Int Arch Allergy Immunol, ():1-17(2025) [pubmed]

10.1159/000549040;

submitter keyword: Human airway epithelial cell line, asthma, Phospho-proteomics,A549, IL-13

Ian P. Lewkowich, PhD
contact affiliation	Division of Immunobiology, Cincinnati Children’s Hospital, Cincinnati, OH Department of Pediatrics, University of Cincinnati, Cincinnati OH Correspondence:
contact email	ian.lewkowich@cchmc.org
lab head
Archana Shankar
contact affiliation	Cincinnati Children's Hospital
contact email	achu.yous@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066489
     1. Label: PRIDE project
     2. Name: Phospho-proteomic analysis of IL-13 treated human airway epithelial cell line (A549)