PXD066486
PXD066486 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Accounting for longitudinal peak quality metrics with MSstats+ enhances differential analysis in proteomic experiments with data-independent acquisition |
| Description | Mass spectrometry-based proteomics with data-independent acquisition benefits from advanced instrumentation and computational analysis. Despite continued im- provements, the quality of quantification may be poor for some measurements. As the scale of proteomic experiments increases, these poor-quality measurements are challenging to characterize by hand, yet they undermine the detection of differentially abundant proteins and the downstream biological conclusions. We introduce MSstats+, a computational workflow that takes as input not only peak intensities reported by data processing tools such as Spectronaut, but also quality metrics such as peak shape and retention time, as well as longitudinal run order profiles of these metrics. MSstats+ translates these metrics into a single measure of quality, and downweights poor quality measurements when detecting differentially abundant proteins. The method offers a natural treatment of missing value imputation, weighting the imputed values according to the quality metrics in the run. We demonstrate the accuracy of the resulting differ- ential analysis, as compared to the standard implementations, in four experiments: two custom benchmarking studies with intentionally induced anomalies, a controlled mix- ture of proteomes, and a large-scale clinical investigation. MSstats+ is implemented in the family of open-source R/Bioconductor packages MSstats, making it accessible for routine and modular use. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-01 |
| AnnouncementXML | Submission_2025-10-01_06:31:27.766.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ozge Karayel |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-23 11:16:48 | ID requested | |
| ⏵ 1 | 2025-10-01 06:31:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Astral, DIA, MSstats, clinical proteomics, CSF, K562, DatasetType:Proteomics |
Contact List
| Ozge Karayel | |
|---|---|
| contact affiliation | Genentech |
| contact email | karayelo@gene.com |
| lab head | |
| Ozge Karayel | |
| contact affiliation | Genentech |
| contact email | karayelo@gene.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098622/ |
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