PXD066447

PXD066447 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of leukemic cells after RNA interference-mediated depletion of CDC25A
Description	Molecular and functional networks driving coordination between cell cycle and mRNA translation remain to be explored. Here, we used mass spectrometry-based proteomics to comprehensively investigate the interactome and phosphoproteome of the cell cycle regulator CDC25A. This highlighted several actors of mRNA regulation, such as RNA-binding proteins and translation factors, as interacting partners of CDC25A. Ectopic overexpression of CDC25A increased global translation without affecting the cell cycle, while inhibiting CDC25 phosphatases rapidly decreased protein synthesis. A Cyclin Dependent Kinase (CDK) interaction-deficient mutant of CDC25A also enhanced translation, indicating a CDK-independent role. Our results revealed an interplay between CDC25A and CDC25B whereby down-regulation of CDC25A led to compensatory overexpression of CDC25B, while down-regulation of CDC25B was not compensated by CDC25A, reducing mRNA translation. Notably, the roles of CDC25A and CDC25B in mRNA translation are independent of the cell cycle, with CDC25A regulating elongation and CDC25B rather involved into initiation. Finally, we confirmed the implication of CDC25A in translation in acute myeloid leukemia cell lines, in which RNA interference-mediated depletion of CDC25A inhibited translation. Altogether, we propose that CDC25 phosphatases can be considered as signaling platforms coordinating cell cycle progression with mRNA translation and protein synthesis.
HostingRepository	PRIDE
AnnounceDate	2026-07-15
AnnouncementXML	Submission_2026-07-15_01:06:27.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilie-Fleur GAUTIER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-23 01:53:23	ID requested
⏵ 1	2026-07-15 01:06:28	announced

10.1038/s44319-026-00852-y;

Shin S, Cargnello M, Pinched, é L, Medale Giamarchi C, Villette C, Gay A, Salnot V, Gautier EF, Dassi E, Millevoi S, Cammas A, Manenti S, Novel CDK-independent function of CDC25 phosphatases in mRNA translation. EMBO Rep, ():(2026) [pubmed]

submitter keyword: cell cycle, mRNA,CDC25A, RNA binding proteins, translation, LC-MS/MS

Stéphane Manenti
contact affiliation	University of Toulouse, INSERM, CNRS, CRCT, France
contact email	stephane.manenti@inserm.fr
lab head
Emilie-Fleur GAUTIER
contact affiliation	Proteom’IC facility, Université Paris Cité, CNRS, INSERM, Institut Cochin, F-75014 PARIS, France
contact email	ef.gautier.upd@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066447
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of leukemic cells after RNA interference-mediated depletion of CDC25A