PXD066425

PXD066425 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass spectrometry study of the adduct formed by F-ALDH with thiram
Description	F-ALDH is a recently characterized psychrophilic aldehyde dehydrogenase from Flavobacterium PL002. Thiram was shown to inhibit its enzymatic activity, yet the mechanism of inhibition is unknown. We have used low flow liquid chromatography (nanoLC) coupled to tandem mass spectrometry (MS/MS) to study the mechanism behind thiram inhibition, as we assumed a Cys-covalent adduct could be formed between F-ALDH and thiram. For this, purified F-ALDH was incubated with thiram and the sample was subjected to Cys reduction or not along a control sample of non-treated F-ALDH.
HostingRepository	PRIDE
AnnounceDate	2025-10-24
AnnouncementXML	Submission_2025-10-24_10:23:06.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Munteanu
SpeciesList	scientific name: Flavobacterium sp. PL002; NCBI TaxID: NEWT:1897058;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-22 12:34:03	ID requested
⏵ 1	2025-10-24 10:23:07	announced

Publication List

10.1016/j.ymeth.2025.10.006;
Ftodiev AI, Petrareanu GN, Puiu M, Proteasa G, Munteanu CVA, Banciu RM, Chauhan R, Visinescu D, Purcarea C, Fanjul-Bolado P, Iba, ñ, ez D, Fogel R, Limson J, Potara M, Bonciu AF, Astilean S, Bala C, Vasilescu A, Comprehensive analysis of the inhibition of aldehyde dehydrogenase from Flavobacterium PL002 and its coupling with SERS as a path for the selective detection of thiram. Methods, 245():83-98(2026) [pubmed]

10.1016/j.ymeth.2025.10.006;

Ftodiev AI, Petrareanu GN, Puiu M, Proteasa G, Munteanu CVA, Banciu RM, Chauhan R, Visinescu D, Purcarea C, Fanjul-Bolado P, Iba, ñ, ez D, Fogel R, Limson J, Potara M, Bonciu AF, Astilean S, Bala C, Vasilescu A, Comprehensive analysis of the inhibition of aldehyde dehydrogenase from Flavobacterium PL002 and its coupling with SERS as a path for the selective detection of thiram. Methods, 245():83-98(2026) [pubmed]

Keyword List

submitter keyword: enzyme inhibition, aldehyde dehydrogenase,mass spectrometry, thiram

submitter keyword: enzyme inhibition, aldehyde dehydrogenase,mass spectrometry, thiram

Contact List

Alina Vasilescu
contact affiliation	International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania
contact email	avasilescu@biodyn.ro
lab head
Cristian Munteanu
contact affiliation	Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	cristian.v.a.munteanu@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066425
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066425

PRIDE project URI

Repository Record List

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