PXD066382 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Asparagine-linked glycosylation mediates glucose sensing for cell fate decision |
| Description | Asparagine-linked glycosylation (N-glycosylation), a fundamental modification of proteins synthesized in the endoplasmic reticulum, is easily suppressed by glucose deprivation, but the role of this metabolic link has long remained unclear. We previously identified the activity of mannose metabolism, which branches from glycolysis, as a key determinant for N-glycosylation efficiency in glucose deprivation. Our genetic approach to uncouple mannose metabolism from glycolysis allowed the precise control of mannose metabolic activity via its salvage pathway, revealing that moderate decrease in N-glycosylation efficiency activated the pro-survival PERK-eIF2 signals, which enabled cells to safely cut metabolic costs in N-glycosylation to the minimal levels required for cell survival. In contrast, severe decrease in N-glycosylation efficiency sensitized lysosomes to external insults, thereby biasing cell fates to cell death. Moreover, we demonstrated the specific role of glucose metabolism in regulating global translation efficiency via LKB1. Our study reveals the unexpected role of N-glycosylation as a glucose-sensing mechanism. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-01_16:30:22.693.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD066382 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Takehiro Suzuki |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-21 21:23:48 | ID requested | |
| ⏵ 1 | 2026-03-01 16:30:23 | announced | |
Publication List
| 10.6019/PXD066382; |
| Wang Z, Miyamoto Y, Suzuki T, Tanaka-Okamoto M, Mizote Y, Dohmae N, Tahara H, Taniguchi N, Harada Y, Mannose metabolic pathway senses glucose supply and regulates cell fate decisions. J Biol Chem, 302(3):111213(2026) [pubmed] |
| 10.1016/j.jbc.2026.111213; |
Keyword List
| submitter keyword: metabolism, cell fate decision,Glucose deprivation, N-glycosylation, unfolded protein response |
Contact List
| Naoshi Dohmae |
|---|
| contact affiliation | Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN |
| contact email | dohmae@riken.jp |
| lab head | |
| Takehiro Suzuki |
|---|
| contact affiliation | RIKEN Center for Sustainable Resource Science |
| contact email | takehirosuzuki@riken.jp |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066382
- Label: PRIDE project
- Name: Asparagine-linked glycosylation mediates glucose sensing for cell fate decision
