PXD066353

PXD066353 is an original dataset announced via ProteomeXchange.

Title	Microglia proteome from the rat substantia nigra. Study in animal model of fluorocitrate-induced astrocyte dysfunction and microglia activation.
Description	Nervous system inflammation and microglia activation are among hallmarks of Parkinson’s disease. Partial nigrostriatal neurodegeneration can be compensated. Interaction between neurons, microglia and astrocytes could be essential for this functional adaptation and neuronal survival in long-term, but which mechanisms are responsible is unknown. The aim was to check how astrocyte vs neuron degeneration affected differentially microglia activation proteome and which mechanisms were regulated. In a rat model of 7-day infusion by osmotic minipumps of fluorocitrate (FC) into the substantia nigra (SN) (doi: 10.1007/s12035-017-0529-z; doi: 10.1111/jnc.14605; doi: 10.1016/j.mito.2018.12.002) astrocytes become dysfunctional and die, strongly activating microglia in the process. 6-OHDA injected into medial forebrain bundle caused selective degeneration of dopaminergic neurons in the SN and only slightly activated microglia, probably via different mechanism. Controls were sham operated. Microglia cells were sorted out from dissociated SN tissue using FACS, and processed for mass spectrometry proteome analysis. Microglial response to dying astrocytes vs dying neurons was different. Understanding the role of individual cell type in the diseased tissue cellular context is essential to understand disease pathomechanisms and identify better pharmacological targets.
HostingRepository	PRIDE
AnnounceDate	2026-02-11
AnnouncementXML	Submission_2026-02-11_01:22:12.000.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066353
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Przemyslaw Mielczarek
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-21 01:42:42	ID requested
⏵ 1	2026-02-11 01:22:12	announced

10.1016/J.DIB.2026.112473;

10.6019/PXD066353;

submitter keyword: Fluorocitrate, Proteomics,Microglia

Przemyslaw Mielczarek
contact affiliation	1) Department of Analytical Chemistry and Biochemisty, AGH University of Krakow 2) Laboratory of Proteomics and Mass Spectrometry, Maj Institute of Pharmacology, Polish Academy of Sciences
contact email	mielcza@agh.edu.pl
lab head
Przemyslaw Mielczarek
contact affiliation	1) AGH University of Krakow, Krakow, Poland 2) Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland
contact email	przemyslaw.mielczarek@agh.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD066353

PRIDE project URI

• PRIDE
  1. PXD066353
     1. Label: PRIDE project
     2. Name: Microglia proteome from the rat substantia nigra. Study in animal model of fluorocitrate-induced astrocyte dysfunction and microglia activation.