PXD066343

PXD066343 is an original dataset announced via ProteomeXchange.

Title	Membrane proteome profile of MSI-tumor organoids exposed to conditioned media of MSI and MSS-tumor organoids
Description	MSI-tumor organoids were previously stimulated with 200 ng/mL IFNγ for 24 hours. Then, organoids were dissociated into single cells using TrypLE express and resuspended in conditioned media. Cells were seeded in a 24-well plate and incubated for 24 hours at 37°C. After incubation, tumor cells were collected, washed in PBS and processed for membrane protein extraction using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Scientific, cat. no. 89842), following the manufacturer’s instructions. Membrane proteome was quantified using the Pierce™ BCA Protein Assay Kits (Thermo Scientific, cat. no. 23225). Then, the membrane protein fraction was digested for LCMSMS analysis.
HostingRepository	PRIDE
AnnounceDate	2026-05-07
AnnouncementXML	Submission_2026-05-07_10:03:06.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	vittoria matafora
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-21 00:44:19	ID requested
⏵ 1	2026-05-07 10:03:07	announced

Dataset with its publication pending

submitter keyword: Protein signature., Mass spectrometry (MS), MSI-tumor organoids,membrane proteomics

angela bachi
contact affiliation	IFOM
contact email	angela.bachi@ifom.eu
lab head
vittoria matafora
contact affiliation	ifom
contact email	vittoria.matafora@ifom.eu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066343
     1. Label: PRIDE project
     2. Name: Membrane proteome profile of MSI-tumor organoids exposed to conditioned media of MSI and MSS-tumor organoids