PXD066334

PXD066334 is an original dataset announced via ProteomeXchange.

Title	SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program
Description	Alternative splicing expands proteomic diversity and is tightly regulated by splicing factors, including the serine/arginine-rich (SR) protein family. Here, we analyze the poorly characterized protein SRSF12. Although SRSF12 is conserved across vertebrates, it is poorly expressed in most mammals, and we find that SRSF12 knockouts in mice do not result in overt physiological or transcriptomic alterations. In contrast, SRSF12 is more highly expressed in primates where it is predominantly expressed during meiosis and in the brain. SRSF12 localizes to splicing speckles in cultured human cells and interacts with the core splicing factors. Strikingly, ectopic expression of SRSF12 in human cells induces widespread transcriptional changes, activating testis- and brain-specific gene expression programs. SRSF12 overexpression also leads to mitotic arrest and cell death, phenotypes that require both its structured RNA recognition motif and intrinsically disordered arginine/serine-rich C-terminal domain. Together, our results suggest that SRSF12 has evolved primate-specific expression to regulate testis- and brain-specific genes.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_16:25:47.236.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066334
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jimmy Ly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-20 14:23:17	ID requested
⏵ 1	2026-01-04 16:25:48	announced

Ly J, Cady SL, Haug S, Khalizeva E, Cheeseman IM, SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program. Mol Biol Cell, 36(11):ar138(2025) [pubmed]

10.6019/PXD066334;

10.1091/mbc.e25-07-0352;

submitter keyword: Splicing, IP-MS, SRSF

Iain Cheeseman
contact affiliation	Whitehead Institute for Biomedical Research and MIT
contact email	icheese@wi.mit.edu
lab head
Jimmy Ly
contact affiliation	PhD student
contact email	jimmyly@wi.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066334
     1. Label: PRIDE project
     2. Name: SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program