PXD066296

PXD066296 is an original dataset announced via ProteomeXchange.

Title	Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry
Description	Haemonchus contortus is a highly pathogenic, blood-feeding nematode that causes widespread disease and significant economic loss in livestock worldwide. Previous proteomic studies were constrained by incomplete genomic resources and limited analytical sensitivity, impeding comprehensive profiling across life stages. In this study, we integrated advanced tandem mass spectrometry with a chromosome-scale genome assembly of the Haecon-5 strain to construct the most detailed somatic proteome of H. contortus to date. We identified and quantified 7,002 proteins across five key developmental stages and sexes (i.e. egg, L3, L4, adult female and adult male), tripling the number identified in previous studies. Comparative analyses revealed pronounced stage-specific expression and functional specialisation, with parasitic stages enriched in proteins linked to metabolism, cellular function and environmental sensing. Fifteen proteins associated with the hypoxia-inducible factor 1 (HIF-1) signalling pathway were upregulated in parasitic stages, suggesting a role in adaptation to hypoxia. Additionally, 150 proteases implicated in haemoglobin degradation were characterised, and functional assays confirmed markedly elevated haemoglobinolytic activity in blood-feeding stages. These findings offer key insights into H. contortus development and parasitism, and establish a high-resolution proteomic framework to underpin fundamental biological studies and enable the discovery of molecular targets for novel interventions against this and related nematodes.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_00:12:31.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-17 22:59:07	ID requested
⏵ 1	2025-09-11 00:12:31	announced

10.1186/S13071-025-07050-0;

submitter keyword: Haemonchus contortus, nematode, LC-MS/MS

Robin Beat Gasser
contact affiliation	Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066296

PRIDE project URI

• PRIDE
  1. PXD066296
     1. Label: PRIDE project
     2. Name: Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry