PXD066253

PXD066253 is an original dataset announced via ProteomeXchange.

Title	Delineating plant responses to the 3’,5’- and 2’,3'-cAMP isomers
Description	Similar to animals, both the 3',5- and the 2'3'-cAMP isomers are present in plants. The former is the enzymatic product of adenylate cyclases (ACs), the latter is an RNA degradation product. While there is increasing evidence that both isomers elicit or modulate (either directly or indirectly) a broad range of physiological responses, the question of isomer specificity of responses has remained largely unresolved. To delineate isomer-specific responses in Arabidopsis thaliana at the systems level, we have combined a comparative proteomics and electrophysiological approaches. Both isomers caused distinct systemic effects on the proteome, with notably the 2',3' isomer affecting systems-level functions like transcriptional regulation. None of the isomers affects net ion fluxes in the root under control conditions, but both were able to attenuate the magnitude of H2O2 net K+ loss and Ca2+ uptake by 2-fold. Isomer-specific responses of single molecular targets were assessed in the cyclic nucleotide-gated channels 2 and 18 (CNGC2 and CNGC18). Both channels are gated by the 3',5'-cAMP isomer only, suggesting that the gating is isomer-specific and, consequently, gating in vivo depends on activated ACs.
HostingRepository	jPOST
AnnounceDate	2026-07-17
AnnouncementXML	Submission_2026-07-16_08:00:12.287.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milena Marsoni
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-17 02:01:36	ID requested
⏵ 1	2026-07-16 08:00:12	announced

Dataset with its publication pending

submitter keyword: Cyclic nucleotide monophosphates, cAMP isomers

Eleonora Davide
lab head
Milena Marsoni
contact affiliation	Università dell'Insubria
dataset submitter

jPOST dataset URI

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