PXD066243

PXD066243 is an original dataset announced via ProteomeXchange.

Title	Multi-omics profiling reveals the role of 4-Ethylbenzoic Acid in promoting proliferation and invasion of cervical cancer
Description	Background: Cervical cancer (CC) is a global health challenge, ranking fourth among cancers in women. Microbiome-metabolome interactions influence human papillomavirus (HPV) associated carcinogenesis, but specific microbial metabolites driving malignant progression remain undefined. This study aimed to identify potential biomarkers for distinguishing CC, and further explore their role in the progression of CC. Methods: Non-targeted metabolomics was employed to profile alterations in the vaginal microenvironment across clinical cohorts, including individuals with CC, individuals with cervical intraepithelial neoplasia (CIN), HPV-positive individuals, and HPV-negative individuals. Targeted metabolomics was then used to confirm the expression of 4-ethylbenzoic acid (4-EA) levels and its role in CC was explored using cell counting kit-8, 5-ethynyl-2'-deoxyuridine, colony formation, transwell, and wound healing assays. Proteomics was used to investigate the effects of 4-EA on CC cells. Results: The metabolic profiles of vaginal secretions in the CC group differed significantly from those in the other three groups. Untargeted metabolomics identified 27 CC-specific metabolites (VIP>2, P<0.05), revealing a marked elevation of 4-EA and its close relationship with vaginal microorganisms. Clinico-pathological correlations revealed progressive 4-EA accumulation across the cervical carcinogenesis stages. Additionally, 4-EA promoted the proliferation, migration, and invasion of CC cells in vitro. Proteomic reprogramming of CC cells following 4-EA treatment identified 14 highly expressed proteins associated with poor prognosis. Conclusions: This multi-omics investigation identified 4-EA as a novel candidate metabolite and a potential biomarker of CC. Identification of key proteins may provide new insights for interventions targeting the development of CC.
HostingRepository	iProX
AnnounceDate	2025-07-17
AnnouncementXML	Submission_2025-10-08_18:03:17.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoling Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 23:35:20	ID requested
⏵ 1	2025-10-08 18:03:17	announced

Dataset with its publication pending

submitter keyword: cervical cancer, 4-ethylbenzoic acid, metabolomics, proteomics, biomarker

Ying Jia
contact affiliation	The First Affiliated Hospital of Chongqing Medical University
contact email	jiaying@hospital.cqmu.edu.cn
lab head
Xiaoling Huang
contact affiliation	The First Affiliated Hospital of Chongqing Medical University
contact email	huangxl9520@163.com
dataset submitter

iProX dataset URI