PXD066207

PXD066207 is an original dataset announced via ProteomeXchange.

Title	The Outer Membrane Vesicle-Associated Peptidyl-arginine Deiminase of Porphyromonas gingivalis is Required for Macrophage Evasion and Modulates Blood-Brain Barrier passage
Description	Porphyromonas gingivalis is a keystone periodontal pathogen implicated in a variety of diseases ranging from periodontitis to Alzheimer’s disease. A hallmark of P. gingivalis is the constitutive production of a peptidyl-arginine deiminase (PPAD), which converts positively charged arginine residues into charge-neutral citrulline residues. The PPAD enzyme is known to be localized to the bacterial outer membrane and outer membrane vesicles (OMVs). Our present study was aimed at dissecting the roles of PPAD in the bacterial evasion of human macrophages and passage of cellular barriers. Here, we show that PPAD is required for activity of the arginine-specific proteases RgpA and RgpB, which are critical virulence factors of P. gingivalis. Furthermore, we show that the absence of PPAD leads to an increased OMV charge. Both changes impact the immune evasion strategy of P. gingivalis, as the PPAD-deficient bacteria display increased biofilm formation while their ability to evade and kill human macrophages is strongly reduced. In fact, PPAD-deficient bacteria and OMVs are more effectively internalized by macrophages and they elicit altered inflammatory macrophage responses, as demonstrated by reduced secretion of pro-inflammatory cytokines and major changes in the macrophage proteome. Intriguingly, we show that P. gingivalis OMVs have a high propensity for in vitro blood-brain barrier passage through transcytosis, which is significantly increased by PPAD-deficiency. This implies that OMV citrullination is a critical determinant for trafficking of P. gingivalis-derived vesicles within the human host. Altogether, our present findings explain how PPAD shapes the immunomodulatory and invasive properties of P. gingivalis and its OMVs.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-14_02:38:36.852.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Porphyromonas gingivalis (strain ATCC BAA-308 / W83); NCBI TaxID: NEWT:242619;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 05:00:19	ID requested
⏵ 1	2026-05-14 02:38:37	announced

10.1016/j.micres.2026.128488;

Xu D, Flores-Vallejo RDC, Qiao M, Liu L, Maa, ß S, Becher D, Wang S, Zuhorn IS, van Dijl JM, The outer membrane vesicle-associated peptidyl-arginine deiminase of Porphyromonas gingivalis is required for macrophage evasion and modulates blood-brain barrier passage in vitro. Microbiol Res, 307():128488(2026) [pubmed]

submitter keyword: macrophages, blood-brain-barrier, PPAD,OMVs, Porphyromonas gingivalis

Dörte Becher
contact affiliation	Porphyromonas gingivalis W83
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD066207

PRIDE project URI

• PRIDE
  1. PXD066207
     1. Label: PRIDE project
     2. Name: The Outer Membrane Vesicle-Associated Peptidyl-arginine Deiminase of Porphyromonas gingivalis is Required for Macrophage Evasion and Modulates Blood-Brain Barrier passage