PXD066194

PXD066194 is an original dataset announced via ProteomeXchange.

Title	Glycosylation Inhibition as a Therapeutic Strategy for Neuroinflammatory Disorders: The Potential of Tanshinone IIA;BV2 cell data
Description	our study employed a combined proteomic and glycoproteomic approach to investigate the role of protein glycosylation in lipopolysaccharide (LPS)-induced neuroinflammation models. Bioinformatics analysis of the proteomic and glycoproteomic data further emphasized a strong connection between our observed changes and pathways implicated in glycosylation and neuroinflammation. Our findings revealed an overall increase in protein glycosylation levels during neuroinflammatory processes. Crucially, we demonstrated that the pan-glycosylation inhibitor NGI-1 effectively suppressed the inflammatory response in BV2 microglia cells. Building on these findings and leveraging the proteomic and glycoproteomic data from LPS-induced models, we subsequently screened Tanshinone IIA (STS), a bioactive component of Salvia miltiorrhiza (Danshen), identifying it as a potent therapeutic candidate for neuroinflammatory disorders. Tanshinone IIA effectively attenuated aberrant protein glycosylation by reducing glycan occupancy. Mechanistically, this modulatory effect likely involves influencing UDP-GlcNAc levels within the glycosylation biosynthesis pathway and impacting N-acetylaspartate metabolism, thereby disrupting aberrant protein glycosylation and consequently alleviating the inflammatory response. Collectively, our findings offer novel insights into the therapeutic potential of targeting glycosylation modifications in neuroinflammation.
HostingRepository	iProX
AnnounceDate	2025-07-16
AnnouncementXML	Submission_2025-07-16_00:26:25.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yahui Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 00:26:11	ID requested
⏵ 1	2025-07-16 00:26:26	announced

Dataset with its publication pending

submitter keyword: Neuroinflammation, Proteomics, Glycosylation proteomics, Glycosylation modification, Tanshinone IIA

Dezhi Kong
contact affiliation	Hebei Medical University
contact email	kongdezhi@hebmu.edu.cn
lab head
Yahui Li
contact affiliation	Hebei Medical University
contact email	1324050219@qq.com
dataset submitter

iProX dataset URI