PXD066187

PXD066187 is an original dataset announced via ProteomeXchange.

Title	Disruption of TEX38 impairs sperm morphogenesis and diverse functions including the migration of sperm into the oviduct
Description	In male reproduction, spermatogenesis and sperm maturation are critical for the production of normal sperm and offspring, yet the underlying molecular mechanisms remain largely elusive. Tex38 is a testis-enriched gene, and its deficiency results in oligoasthenoteratospermia (OAT) with aberrant epididymis, leading to male infertility in mice. Tex38 knockout (KO) sperm primarily exhibited neck bending deformities and functional abnormalities, including impaired fertilization. Proteomic analysis identified ADAM3 and its maturation-associated chaperones (CALR3, CLGN, and PDILT) as the most significantly altered proteins among the differentially expressed proteins (DEPs) in both sperm and epididymis of Tex38 knockout mice. GO analysis revealed that DEPs were primarily involved in sperm morphogenesis, motility, and fertilization. ARRDC5 was identified as a novel interacting protein of TEX38, and its deletion resulted in similar male infertility phenotypes as Tex38 deletion. Immunoprecipitation-mass spectrometry identified TEX38 and ARRDC5 interacts with CLGN and PDILT. The interactions among TEX38, ARRDC5, PDILT, and CLGN were found to affect ADAM3 maturation, resulting in the failure of both Tex38-/- and Arrdc5-/- sperm to migrate to the oviduct. Overall, these findings establish TEX38 as an essential regulator of mammalian ADAM3-related migration, sperm formation, energy metabolism (ATP generation) sperm-egg binding, and fertilization. TEX38 represents a potential target for diagnosis and treatment of male infertility and male contraception.
HostingRepository	iProX
AnnounceDate	2025-07-15
AnnouncementXML	Submission_2025-07-15_20:08:57.165.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lu Yuan
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 20:08:42	ID requested
⏵ 1	2025-07-15 20:08:57	announced

Dataset with its publication pending

submitter keyword: TEX38, male infertility, ARRDC5, sperm migration, ADAM3 maturation, sperm morphogenesis

Ying Zheng
contact affiliation	School of Medicine, Yangzhou University
contact email	yzzkl@163.com
lab head
Lu Yuan
contact affiliation	School of Medicine, Yangzhou University
contact email	yuanlu0217@163.com
dataset submitter

iProX dataset URI