PXD066185
PXD066185 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of potential ICAM3-interacting proteins using IP-MS |
| Description | IP-MS assays were used to identify the protein(s) that might interact with ICAM3. Briefly, the THP1-Mφ stably expressing Flag-ICAM3 were co-cultured with WT authentic SARS-CoV-2-infected A549-ACE2 cells for 48 h. After co-culture, THP1-Mφ were lysed in NETN buffer and immunoprecipitated using anti-Flag M2 agarose (M8823; Sigma-Aldrich, MO, USA). After washing four times with the high stringent lysis buffer, the immunoprecipitants were resolved on gradient SDS-polyacrylamide gel electrophoresis (PAGE), and the gel bands were processed for mass spectrometry. Proteins were reduced with dithiothreitol, alkylated with iodoacetamide, and digested with trypsin. Peptides were extracted, vacuum-dried, and reconstituted in formic acid for MS analysis using an Orbitrap Fusion spectrometer. The tandem mass spectra were searched against the human UniProt database (version 20140922, containing 20,193 sequences) using MaxQuant (version 1.5.3.30). The false discovery rates (FDR) of the peptide-spectrum matches (PSMs) and proteins were set to less than 1%. |
| HostingRepository | iProX |
| AnnounceDate | 2025-07-15 |
| AnnouncementXML | Submission_2025-07-15_20:06:17.094.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuanfeng Li |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-15 20:06:00 | ID requested | |
| ⏵ 1 | 2025-07-15 20:06:17 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: IP-MS, ICAM3 |
Contact List
| Gangqiao Zhou | |
|---|---|
| contact affiliation | State Key Laboratory of Proteomics, National Center for Protein Sciences at Beijing, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine |
| contact email | zhougq114@126.com |
| lab head | |
| Yuanfeng Li | |
| contact affiliation | State Key Laboratory of Proteomics, National Center for Protein Sciences at Beijing, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine |
| contact email | liyf_snp@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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