PXD066175

PXD066175 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome Profiling in the Rat Dorsal Striatum Synaptoneurosome Following Methamphetamine Exposure
Description	We performed discovery-based proteomic profiling of synaptoneurosomes (SNs), subcellular fractions of brain tissue containing both pre- and post-synaptic components, isolated from the dorsal striatum of rats following methamphetamine (Meth) administration to investigate how methamphetamine exposure alters the synaptic proteome. Using tandem mass tag (TMT)-based multiplexed quantification with high-resolution mass spectrometry (HRMS), we identified 6094 proteins and quantified 4583 in 3 out 5 biological replicates. Comparative analysis between Meth-treated and control rats revealed differential regulation for 147 proteins. These proteins were enriched in synaptic, mitochondrial, and proteasomal pathways.
HostingRepository	PRIDE
AnnounceDate	2025-11-19
AnnouncementXML	Submission_2025-11-19_15:43:52.665.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066175
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 09:14:41	ID requested
⏵ 1	2025-11-19 15:43:53	announced

Publication List

10.6019/PXD066175;
10.1021/ACSCHEMNEURO.5C00679;

10.6019/PXD066175;

10.1021/ACSCHEMNEURO.5C00679;

Keyword List

submitter keyword: synaptoneurosome, proteomics,dorsal striatum, mass spectrometry, methamphetamine

submitter keyword: synaptoneurosome, proteomics,dorsal striatum, mass spectrometry, methamphetamine

Contact List

Peter Nemes
contact affiliation	Department of Chemistry and Biochemistry, Univeristy of Maryland, Maryland, USA
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD066175
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD066175

PRIDE project URI

Repository Record List

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