PXD066173 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation |
| Description | Blood plays a vital role in diagnostic medicine by providing essential information for assessing health and diagnosing various conditions. Routine blood tests can reveal important biomarkers that support early diagnosis and timely intervention. Recently, extracellular vesicles (EVs) have emerged as promising biomarkers due to their role in intercellular communication and involvement in disease processes such as cancer and neurodegeneration. However, isolating EVs from plasma remains challenging, as residual blood cells, especially platelets, can interfere with vesicle release. In this study, we explore the use of the PRotein Organic Solvent PRecipitation (PROSPR) method, a straightforward and effective technique previously applied to plasma and brain samples, to isolate EVs directly from whole blood. We performed detailed analyses, including ultrastructural imaging, cytometry, and multi-omics profiling, to characterize the EVs obtained. Our findings demonstrate that PROSPR can successfully capture even the smallest exosomes (30–120 nm), which are often missed in plasma-based isolation. This approach represents a significant advancement in EV research and may enhance the identification of blood-based biomarkers for a range of diseases. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_01:51:32.038.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Aida Serra |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Bruker Daltonics timsTOF series |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-15 08:15:22 | ID requested | |
| ⏵ 1 | 2026-08-25 01:51:32 | announced | |
Publication List
Keyword List
| submitter keyword: Whole blood, Human,Extracellular vesicles, PROSPR |
Contact List
| Aida Serra Maqueda |
|---|
| contact affiliation | Biomedical Research Institute of Lleida Dr. Pifarré Foundation (IRBLLEIDA) - +Pec Proteomics Research Group (+PPRG) - Neuroscience Area – University Hospital Arnau de Vilanova (HUAV), 80 Av. Rovira Roure, 25198, Lleida, Spain Department of Medical Basic Sciences, University of Lleida (UdL), +Pec Proteomics Research Group (+PPRG), 25198, Lleida, Spain |
| contact email | aida.serra@udl.cat |
| lab head | |
| Aida Serra |
|---|
| contact affiliation | Department of Medical Basic Sciences, University of Lleida (UdL) - Biomedical Research Institute of Lleida Dr. Pifarré Foundation (IRBLLEIDA), +Pec Proteomics Research Group (+PPRG), 25198, Lleida, Catalonia, Spain |
| contact email | aida.serra@udl.cat |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD066173 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066173
- Label: PRIDE project
- Name: Characterization of Whole Blood EVs Using PRotein Organic Solvent PRecipitation
