PXD066171

PXD066171 is an original dataset announced via ProteomeXchange.

Title	Chlorella vulgaris Protein Isolate Effectively Protects Lacticaseibacillus rhamnosus GG Viability During processing, Storage, and In vitro Digestion
Description	This study examined the protective effect of Chlorella vulgaris protein isolate (CPI) on the biological activity of Lacticaseibacillus rhamnosus GG (LGG) during lyophilisation, storage, and in vitro digestion. Prior to lyophilisation, probiotic hydrogels were either fermented to pH 4.5 (CF) or left untreated (CNT). The microstructure, physicochemical, and thermal properties of the resulting probiotic powders were analysed, along with LGG adhesion to an in vitro intestinal epithelium model. Embedment in CPI-based matrices provided effective lyoprotection, with CNT offering greater stability than CF. Elevated storage temperatures and relative humidity (RH) accelerated LGG inactivation, particularly in CF. While a sufficient proportion of LGG survived the harsh conditions during in vitro digestion, pre-fermentation had a negligible impact. Additionally, a satisfactory level of LGG adhesion to the intestinal mucus layer was observed. Overall, CPI exhibits strong stabilizing properties for LGG viability and represents a promising plant-based alternative to dairy proteins for probiotic food formulations.
HostingRepository	PRIDE
AnnounceDate	2026-01-06
AnnouncementXML	Submission_2026-01-06_02:39:17.285.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066171
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: NEWT:3077; scientific name: Lactobacillus rhamnosus GG; NCBI TaxID: NEWT:568703;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 07:46:44	ID requested
⏵ 1	2026-01-06 02:39:17	announced

10.6019/PXD066171;

10.1016/J.FOODHYD.2025.111999;

submitter keyword: Probiotics, Encapsulation,Microalgae, Protein isolate, Viability, In vitro digestion

Leclercq Celine
contact affiliation	LIST_Environmental and industrial biotechnologies Bioprocessing and formulation L-4940, BASCHARAGE LUXEMBOURG
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST - Environmental and Industrial Biotechnologies
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066171
     1. Label: PRIDE project
     2. Name: Chlorella vulgaris Protein Isolate Effectively Protects Lacticaseibacillus rhamnosus GG Viability During processing, Storage, and In vitro Digestion