PXD066161

PXD066161 is an original dataset announced via ProteomeXchange.

Title	Linking First-Pass Reperfusion Success to Proteomic Markers in Large and Medium Vessel Ischemic Stroke
Description	Mechanical thrombectomy (MT) has improved disability outcomes after ischemic stroke (IS), mainly when the so-called modified first-pass effect (mFPE) is achieved. This study aims to explore the proteomic profiling of thrombi composition in those patients who achieved mFPE. A prospective study was conducted based on consecutive IS patients who underwent MT for proteomic analysis of the thrombi, comparing whether the mFPE was achieved or not. A qualitative and quantitative protein analysis was performed using LC-MS/MS for DDA and SWATH-MS analysis, respectively. Dysregulated proteins were considered when the range p-value <0.05, and a fold change >1.5 (upregulated) or <0.6 (downregulated). A total of 88 patients were included, with a median age of 75 years (±16) and 55.7% females (49 patients). mFPE was presented in 48.9% (43 patients), and showed better functional independence at 90 days (62.2% vs. 37.8%; p=0.02). Proteomic qualitative DDA analysis showed 9 dysregulated proteins, while quantitative SWATH-MS analysis found 79 dysregulated proteins, both for patients who achieved mFPE (ACTN1, ACTN4, ANXA1, HSP90AB1, HSP90B1, PPBP, HBA1, FLNA, TLN1, SCPEP1, LGALS1, ADAP1, ITGB3, and S100A9). The most relevant biological pathways involved were endothelial dysfunction, platelet dysfunction, atheromatosis, and inflammation. This proteomic study provides new insights into the biological complexity of IS, revealing distinct protein expression patterns, particularly in those who achieved the mFPE, and may serve as potential biomarkers for prognostication and therapeutic targeting.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-29_16:43:33.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 04:53:41	ID requested
⏵ 1	2026-03-29 16:43:34	announced

10.1093/pcmedi/pbag008;

Oblitas CM, Fern, á, ndez-Rodicio S, Rodr, í, guez-Y, á, ñ, ez M, Rodr, í, guez-Castro E, Gonzalez-Quintela A, Porto-, Á, lvarez J, Mart, í, nez-Fern, á, ndez J, Blanco M, Taboada JL, Martnez-Reiriz S, Bravo SB, Pena C, Castillo J, Alonso-Alonso ML, Hervella P, Mosqueira AJ, Iglesias-Rey R, Linking first-pass reperfusion success to proteomic markers in large and medium vessel ischemic stroke: an exploratory study. Precis Clin Med, 9(1):pbag008(2026) [pubmed]

submitter keyword: biological pathways

ischemic stroke

mechanical thrombectomy

proteomics

thromboinflammation

Susana B Bravo
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD066161

PRIDE project URI

• PRIDE
  1. PXD066161
     1. Label: PRIDE project
     2. Name: Linking First-Pass Reperfusion Success to Proteomic Markers in Large and Medium Vessel Ischemic Stroke