PXD066158

PXD066158 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of CLDN6-deficient germ cell tumor cells
Description	Representing a crucial factor during cell adhesion, polarity, proliferation and migration, the transmembrane protein claudin 6 (CLDN6) has been shown to be a putative target for the treatment of germ cell tumors (GCT). Particularly seminoma (SE), embryonal carcinoma (EC), and choriocarcinoma (CC) indicated high CLDN6 levels, while about 60 % of yolk-sac tumors (YST) presented as CLDN6+. In this study, we generated CLDN6-deficient GCT cells by CRISPR/Cas9 for SE, EC, and CC cells, while YST cells were cell sorted for CLDN6+ and CLDN6- populations. Further, the putative function of CLDN6 was investigated on proteome level using liquid-chromatography coupled with mass spectrometry (LS-MS).
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_08:04:20.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 03:26:01	ID requested
⏵ 1	2026-04-07 08:04:21	announced

Zwick J, Skowron MA, Poschmann G, Petzsch P, Bergmann AK, St, ü, hler K, Hecht J, Nettersheim D, The Functional Role and Molecular Characterization of the Therapeutic Target CLDN6 in Germ Cell Tumors. Andrology, ():(2026) [pubmed]

10.1111/andr.70217;

submitter keyword: Germ cell tumor, seminoma, embryonal carcinoma, choriocarcinoma, yolk-sac tumor, CLDN6

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine Heinrich Heine University Düsseldorf Universitätsstraße 1 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD066158

PRIDE project URI

• PRIDE
  1. PXD066158
     1. Label: PRIDE project
     2. Name: Proteomic profiling of CLDN6-deficient germ cell tumor cells