PXD066147

PXD066147 is an original dataset announced via ProteomeXchange.

Title	Rejuvenation of mesenchymal stem cells by human peripheral blood lymphocytes-A dual-mechanism for targeting senescent cell clearance and promoting cell proliferation in a coculture system
Description	Here we presented a novel approach to combat cellular senescence and promote cell proliferation by coculturing human MSCs with human peripheral blood lymphocytes (PBLs). In a heterogeneous population of MSCs comprising both aged and nonaged cells, PBL effector cells, rather than their cytokines, exhibited a dual role. They selectively induced apoptosis in aged cells by facilitating p53 SUMOylation and activating the p53-dependent mitochondrial pathway, while simultaneously safeguarding younger cells against senescence and promoting cell proliferation by activating Serpinb2/NF-κB signaling. This resulted in a decrease in aged MSCs and an enrichment of rejuvenated MSCs. This process effectively reversed the senescence phenotype, leading to the remodeling of stemness characteristics and the enhancement of functionality within the MSC population. Furthermore, MSCs rejuvenated by PBLs presented an enhanced therapeutic efficacy and a favorable safety profile.
HostingRepository	iProX
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-15_01:08:15.652.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Luo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 01:07:56	ID requested
⏵ 1	2025-07-15 01:08:16	announced

Dataset with its publication pending

submitter keyword: Mesenchymal stem cell, Human peripheral blood lymphocytes

Hui Jian
contact affiliation	Affiliated Hospital of Zunyi Medical University
contact email	jhxiao@zmu.edu.cn
lab head
Yi Luo
contact affiliation	Affiliated Hospital of Zunyi Medical University
contact email	luoyi199209@126.com
dataset submitter

iProX dataset URI