PXD066145

PXD066145 is an original dataset announced via ProteomeXchange.

Title	DrPISA: Deep Eutectic Solvent-Assisted Reverse Proteome-Integrated Solubility Alteration for High-Sensitivity Drug Target Identification
Description	Existing thermal shift-based mass spectrometry approaches, such as TPP and PISA, focus mainly on soluble fractions, often neglecting the insoluble pellet, limiting comprehensive drug–target profiling. In this work, we developed DrPISA (deep eutectic solvent-assisted reverse PISA), a novel strategy that enables direct analysis of protein precipitates by integrating optimized deep eutectic solvents (DESs) into the PISA framework. Combined with LC-MS/MS, DrPISA demonstrated broad applicability across multiple model compounds, including methotrexate (MTX), cyclosporin A (CsA), geldanamycin (GA), panobinostat (PAN), and staurosporine (STS), as well as a natural product celastrol (CEL), highlighting its potential in target identification for bioactive natural products.
HostingRepository	iProX
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-15_01:06:17.570.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	dimethylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 01:06:01	ID requested
⏵ 1	2025-07-15 01:06:17	announced

Dataset with its publication pending

submitter keyword: Proteome integral solubility alteration

Deep eutectic solvent

Protein solubilization agent

Drug target discovery

Celastrol.

Wen Gao
contact affiliation	China Pharmaceutical University
contact email	gw_cpu@126.com
lab head
Liu Yang
contact affiliation	China Pharmaceutical University
contact email	m15735653961@163.com
dataset submitter

iProX dataset URI