PXD066141

PXD066141 is an original dataset announced via ProteomeXchange.

Title	Identification of pre-miRNA156a binding proteins by MCP-MS2/TurboID
Description	This study developed an integrated MCP-MS2/TurboID proximity labeling system to identify pre-miRNA156a-interacting proteins in vitro. By co-expressing MS2-tagged pre-miRNA156a with MCP-TurboID fusion protein, we achieved specific recruitment of TurboID to the target RNA. Following a 10-minute biotin pulse, proximal proteins were biotinylated, enriched using streptavidin magnetic beads, and separated by SDS-PAGE. Comparative analysis of differential protein bands between experimental and control groups, followed by LC-MS/MS analysis of excised gel bands (35-55 kDa), enabled systematic identification of pre-miRNA156a-binding proteins. This approach combines the specificity of MCP-MS2 interaction with the sensitivity of TurboID-mediated proximity labeling for comprehensive RNA-protein interactome profiling.
HostingRepository	iProX
AnnounceDate	2025-07-15
AnnouncementXML	Submission_2025-07-15_00:59:34.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Chen
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 00:59:20	ID requested
⏵ 1	2025-07-15 00:59:34	announced

Dataset with its publication pending

submitter keyword: RNA-protein interaction, Proximity labeling, Pre-miRNA156a

Yunfeng Liu
contact affiliation	Guangxi University
contact email	today55955@126.com
lab head
Wei Chen
contact affiliation	Guangxi University
contact email	chenw0425@163.com
dataset submitter

iProX dataset URI