PXD066140
PXD066140 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In situ identifying sennoside A-reducing bacteria guilds in human gut microbiota via enzymatic activity visualization |
| Description | We developed an enzymatic activity visualization platform that uses active intracellular enzymes as molecular baits to capture specific substrate probes. This platform links enzyme-mediated biochemical activity to their in situ localization, isolation, phylogenetic identification, and functional validation. Combined with a highly specific alkyne-azide cycloaddition reaction, these probes enable fluorescent visualization of strains harboring isozymes in human gut microbiota under anaerobic conditions. By performing metabolic capacity tests, fluorescence imaging, and proteomic analyses on four positive and three negative reference strains, we validated that our probes exhibit selectivity at the bacterial cell level. Using the platform, we successfully identified functional guilds involved in the reduction of sennoside A, a widely used laxative prodrug activated by gut bacteria. |
| HostingRepository | iProX |
| AnnounceDate | 2025-07-15 |
| AnnouncementXML | Submission_2025-07-15_00:57:45.331.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chuanjia Zhai |
| SpeciesList | scientific name: Clostridium butyricum; NCBI TaxID: 1492; scientific name: [Ruminococcus] gnavus E1; NCBI TaxID: 935582; scientific name: Streptococcus thermophilus; NCBI TaxID: 1308; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-15 00:57:32 | ID requested | |
| ⏵ 1 | 2025-07-15 00:57:45 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: functional guilds, enzymatic activity visualization, substrate-based probes, FACS, sennoside A, nitroreductase |
Contact List
| Xiaoyan Gao | |
|---|---|
| contact affiliation | Beijing University of Chinese Medicine |
| contact email | gaoxiaoyan@bucm.edu.cn |
| lab head | |
| Chuanjia Zhai | |
| contact affiliation | Beijing University of Chinese Medicine |
| contact email | zcj759869@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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