PXD066133

PXD066133 is an original dataset announced via ProteomeXchange.

Title	Salivary Proteomics Reveals Oxidative and Inflammatory Markers in E-Cigarette Users
Description	Electronic cigarettes, also known as ecig-s, have been widely used in recent years, especially among younger populations. Cytotoxic and carcinogenic substances can be present in their composition, but little is known about the risks associated with their use. The purpose of this study was to evaluate the proteome profile of saliva from users of electronic cigarettes. Participants were divided into two groups: the Electronic Cigarette Group (EG), consisting of 25 regular and exclusive e-cig users, and the Control Group (CG), comprising 25 non-smokers and non-e-cig users, matched by sex and age to the EG. All participants underwent clinical examination and unstimulated saliva collection for evaluation of the salivary proteome. Shotgun proteomics resulted in the identification of 168 proteins, including 48 exclusive to EG, 28 exclusive to CG, and 92 shared by both groups. Statistical comparison (t-test) between EG and CG revealed four differentially expressed proteins: Fatty acid binding protein 5, Calgranulin-B, and Cornifin-A in the CG, and cytostatin-D in the EG. Additionally, a higher abundance of proteins was observed, including glutathione S-transferase P, Immunoglobulin J chain and Acetyl-CoA dehydrogenase, in the EG group. These findings suggest an organismal response to increased oxidative stress, the presence of inflammatory components, and altered salivary microbiome, which can predispose individuals to the development of future pathological conditions.
HostingRepository	PRIDE
AnnounceDate	2026-03-10
AnnouncementXML	Submission_2026-03-10_11:41:24.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Zelanis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-14 20:30:24	ID requested
⏵ 1	2026-03-10 11:41:25	announced

10.1021/ACS.JPROTEOME.5C00347;

submitter keyword: Proteome

Saliva

Electronic Cigarette Uses

Electronic Nicotine Delivery Systems

Glutathione Transferase.

Andre Zelanis
contact affiliation	Federal University of Sao Paulo, Functional Proteomics Laboratory, Sao Jose dos Campos, Sao Paulo, Brazil.
contact email	andre.zelanis@unifesp.br
lab head
Andre Zelanis
contact affiliation	Laboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil
contact email	zelanis@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD066133

PRIDE project URI

• PRIDE
  1. PXD066133
     1. Label: PRIDE project
     2. Name: Salivary Proteomics Reveals Oxidative and Inflammatory Markers in E-Cigarette Users