PXD066123
PXD066123 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protocol for structural analysis of amyloid fibrils using hydrogen/deuterium exchange mass spectrometry |
| Description | Amyloid fibrils are common in many diseases. This protocol uses α-synuclein fibrils, a Parkinson’s disease hallmark, to demonstrate hydrogen-deuterium exchange mass spectrometry (HDX-MS) for structural analysis. It details fibril preparation, quality assessment, HDX labeling, digestion, and mass spectrometry. Steps for peptide mapping and data interpretation are included. This workflow serves as a practical guide for researchers, particularly new comers, to study insoluble protein aggregates with distinct conformations. |
| HostingRepository | iProX |
| AnnounceDate | 2025-07-14 |
| AnnouncementXML | Submission_2025-07-14_19:11:37.927.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qian Meng |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-14 19:11:20 | ID requested | |
| ⏵ 1 | 2025-07-14 19:11:38 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Protocol, hydrogen/deuterium exchange mass spectrometry |
Contact List
| Hu Zhou | |
|---|---|
| contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
| contact email | zhouhu@simm.ac.cn |
| lab head | |
| Qian Meng | |
| contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
| contact email | mqfly0623@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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