PXD066118

PXD066118 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	System-wide profiling of pre-miRNA156a interacting proteins by MCP-MS2/TurboID
Description	This project presents a comprehensive profiling of pre-miRNA156a-interacting proteins using an integrated MCP-MS2 and TurboID proximity labeling approach in vitro. We performed fusion expression of pre-miRNA with MS2, and co-expressed MCP-TurboID. Due to the specific binding between MCP and MS2, TurboID is brought into close proximity with pre-miRNA. Upon exogenous addition of biotin, interacting molecules of pre-miRNA are biotinylated by TurboID. After cell lysis and incubation with streptavidin magnetic beads, the biotinylated proteins are enriched. Finally, mass spectrometry (MS) analysis is performed to identify the molecules interacting with pre-miRNA, thereby capturing the interaction profile of pre-miRNA-associated proteins.
HostingRepository	iProX
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-14_19:02:52.096.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Chen
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-14 19:02:34	ID requested
⏵ 1	2025-07-14 19:02:52	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: RNA-protein interaction, Proximity labeling, Pre-miRNA156a

submitter keyword: RNA-protein interaction, Proximity labeling, Pre-miRNA156a

Contact List

Full Dataset Link List

iProX dataset URI