PXD066107

PXD066107 is an original dataset announced via ProteomeXchange.

Title	: The Tip60 acetylome is a hallmark of the proliferative state in Drosophila
Description	The acetyltransferase KAT5/Tip60 is a key epigenetic regulator of transcription and the DNA damage response. In Drosophila, Tip60 functions within the DOM-A complex to acetylate histones, but its broader substrate repertoire and cellular roles remain incompletely defined. Here, we comprehensively investigated the functions of Tip60 in a proliferative Drosophila cell model. Tip60 depletion caused cell cycle arrest, yet surviving cells exhibited resistance to mutagenic irradiation. This impaired proliferation was accompanied by reduced expression of critical cell cycle regulators, with Tip60 binding at their transcription start sites and Tip60-dependent acetylation of the histone variant H2A.V correlating with transcriptional activation. Beyond chromatin, Tip60 was found to acetylate a wide range of nuclear proteins involved in replication, mitosis, gene expression, chromatin organization, and ribosome biogenesis. These findings suggest that Tip60 coordinates the proliferative state through the combined regulation of histone and non-histone substrates. We propose that reversible acetylation of diverse effector proteins provides a mechanism for dynamically adjusting growth-related processes in response to cellular stress or nutrient availability. Collectively, this work identifies the DOM-A/Tip60 complex as a general promoter of cell proliferation.
HostingRepository	PRIDE
AnnounceDate	2025-07-16
AnnouncementXML	Submission_2025-07-16_12:26:52.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-14 12:51:19	ID requested
⏵ 1	2025-07-16 12:26:53	announced

Dataset with its publication pending

submitter keyword: acetylome, Drosophila,Tip60, proliferation

Prof. Dr. Peter Becker
contact affiliation	Molecular Biology Biomedical Center Munich Ludwig-Maximilians-University of Munich Großhaderner Strasse 9 82152 Planegg-Martinsried (Germany) Phone: (089) 2180 75427 Fax: (089) 2180 75425
contact email	pbecker@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD066107

PRIDE project URI

• PRIDE
  1. PXD066107
     1. Label: PRIDE project
     2. Name: : The Tip60 acetylome is a hallmark of the proliferative state in Drosophila