⮝ Full datasets listing
PXD066074
PXD066074 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Detection and Characterization of Protein Methylation in Bacteriophages and Their Host, Cellulophaga baltica, During Infection |
| Description | Bacteria and their viruses (bacteriophages or phages) are in a dynamic arms race that balances predation and resistance, each deploying various strategies, including protein post-translational modifications (PTMs), to achieve dominance. To better understand the role PTMs play in phage infection, we infected Cellulophaga baltica bacteria with three previously characterized phages that represent diverse genomes and infection efficiencies (phi18:1, phi18:4, and phi38:1) to identify proteome-wide and protein-specific trends of PTMs. Approximately double the number of methylated residues on proteins were detected in phage-infected cells (virocells) compared to uninfected cells, and significantly increased frequencies of protein methylation were observed during the early stages of infection. This notable result led to a focus on protein methylation. Phage proteins were detectably methylated in both virocells and free virions, neither of which has been previously reported. Host proteins with known importance to phage infection--including GTPase EF-Tu, chaperone DnaK, and gliding motility proteins--were frequently methylated and/or exhibited methylation patterns in virocells that contrasted those in uninfected cells. Collectively, our results expand on the growing interest of the important role PTMs play in phage infection by demonstrating the dynamic methylation of phage proteins as well as host proteins important to phage infection. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-27 |
| AnnouncementXML | Submission_2026-02-27_13:38:05.327.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Andrew Stai |
| SpeciesList | scientific name: Cellulophaga baltica; NCBI TaxID: 76594; scientific name: Cellulophaga baltica phage phi18:1; scientific name: Cellulophaga baltica phage phi38:1; scientific name: Cellulophaga baltica phage phi18:4; |
| ModificationList | unknown modification; unknown modification; unknown modification; unknown modification |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-11 13:57:54 | ID requested | |
| ⏵ 1 | 2026-02-27 13:38:05 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Phage, Proteomics, Virocell, Methylation, Bacteriophage, Infection, Post-translational modification, DatasetType:Proteomics |
Contact List
| Dr. Robert Hettich | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | hettichrl@ornl.gov |
| lab head | |
| Andrew Stai | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | astai@vols.utk.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098485/ |
