PXD066058

PXD066058 is an original dataset announced via ProteomeXchange.

Title	Platelet carbamylated protein LC-MS/MS
Description	Urea undergoes natural hydrolysis to form cyanate, a reactive species capable of inducing protein carbamylation. To determine whether elevated urea levels promote carbamylation of platelet proteins, whether this modification is mediated by the urea transporter B (UT-B) expressed on platelets, and which specific proteins are affected, we employed immunoprecipitation coupled with data-independent acquisition (DIA) mass spectrometry to profile carbamylated proteins. Platelets isolated from wild-type or UT-B knockout mice were incubated with urea or a control solvent. Subsequently, the platelets were lysed with RIPA, and carbamylated proteins were enriched via agarose beads conjugated with anti-carbamylated protein antibodies. Following on-bead enzymatic digestion, DIA mass spectrometric analysis was performed using an Orbitrap Astral mass spectrometer interfaced with the Vanquish Neo UHPLC system. These data represent the quantitative profiles of carbamoylated proteins detected by DIA mass spectrometry. The samples were categorized into four experimental groups: wild-type mouse platelets treated with solvent control (Ctrl), WT mouse platelets treated with urea (U), UT-B knockout mouse platelets treated with solvent control (PU), and UT-B knockout mouse platelets treated with urea stimulation (UPU), with three biological replicates per group.
HostingRepository	PRIDE
AnnounceDate	2026-05-28
AnnouncementXML	Submission_2026-05-27_21:42:20.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiwei Qiu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	carbamoylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-11 08:01:05	ID requested
⏵ 1	2026-05-27 21:42:20	announced

Dataset with its publication pending

submitter keyword: Carbamylation, Platelet, Urea transporter B

Qian Xiang
contact affiliation	Institute of Clinical Pharmacology, Peking University First Hospital, China
contact email	xiangqz@126.com
lab head
Zhiwei Qiu
contact affiliation	Peking University First Hospital
contact email	11067@pkufh.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066058
     1. Label: PRIDE project
     2. Name: Platelet carbamylated protein LC-MS/MS