PXD066009

PXD066009 is an original dataset announced via ProteomeXchange.

Title	Bovine colostrum-derived extracellular vesicles impair cancer cell proliferation through transcriptional repression.
Description	Milk-derived extracellular vesicles (EVs) are a promising source of molecules with therapeutic potential. Bovine colostrum is particularly enriched in EVs, which carry a unique cargo of proteins involved in immune regulation, development, and cellular signaling. However, despite promising bioactive effects in various fields, little is known about their potential as anti-cancer agents. Here, we demonstrate that colostrum-derived EVs (Col-EVs) exert a potent and specific anti-proliferative effect on gastrointestinal cancer cell lines, independent of apoptosis induction. Using a multi-modal approach combining proteomics and functional assays, we show that Col-EVs induce a reversible proliferative arrest through transcriptional repression and chromatin and nuclear remodeling. Col-EV treatment leads to widespread dysregulation of RNA processing and transcriptional machinery, including the downregulation of splicing factors and chromatin regulators essential for cell cycle progression. These molecular changes are accompanied by chromatin compaction, nuclear reorganization, and cytoskeletal remodeling. Notably, these effects do not induce cell death and are reversible upon EV removal, suggesting a modulatory mechanism rather than cytotoxicity. Furthermore, Col-EVs enhance the efficacy of DNA-targeting chemotherapies such as 5-fluorouracil, indicating their potential as adjunctive agents in cancer treatment. Overall, our findings reveal that Col-EVs can selectively and reversibly suppress cancer cell proliferation by reprogramming transcriptional and nuclear architecture, offering a natural, biocompatible strategy for modulating tumor growth and sensitizing cancer cells to conventional therapies.
HostingRepository	PRIDE
AnnounceDate	2026-09-10
AnnouncementXML	Submission_2026-09-10_03:02:30.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-10 06:08:54	ID requested
⏵ 1	2026-09-10 03:02:31	announced

10.1002/JEV2.70361;

submitter keyword: Bovine colostrum, cell growth arrest, extracellular vesicles, chromatin remodeling, gastrointestinal cancer, transcriptional repression, proteomics.

Susana B Bravo
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066009
     1. Label: PRIDE project
     2. Name: Bovine colostrum-derived extracellular vesicles impair cancer cell proliferation through transcriptional repression.