⮝ Full datasets listing
PXD065972
PXD065972 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteins co-immunoprecipitated with the COPI complex |
| Description | WI-38 fibroblasts were lysed in RIPA buffer supplemented with protease/phosphatase inhibitor (Thermo Fisher Scientific), incubated on ice for 10 minutes, and centrifuged for 20 minutes at 13,200 rpm and 6 degrees Celsius. The supernatant was collected, and 1 mg of protein lysate was incubated with 10 ug of antibody (COPB1, COPE, COPZ1, all from Protein Tech, and IgG from Cell Signaling Technology) and 50 uL of DynabeadsTM protein G (Thermo Fisher Scientific 10004D). After overnight incubation at 4 degrees Celsius on a spinning rotor the beads were washed 5 times with RIPA buffer. Immunoprecipitated samples were processed for in-solution trypsin/LysC digestion and analyzed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) (Poochon Scientific). The resulting MS data was searched against the human protein sequence database using Proteome Discoverer 2.5 (Thermo Fisher Scientific). |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-10 |
| AnnouncementXML | Submission_2026-06-10_14:14:48.083.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Krystyna Mazan-Mamczarz |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-09 19:51:09 | ID requested | |
| ⏵ 1 | 2026-06-10 14:14:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: COPI coatomer complex, Golgi apparatus, intracellular trafficking, DatasetType:Proteomics |
Contact List
| Myriam Gorospe | |
|---|---|
| contact affiliation | National Institute on Aging, Intramural Research Program, National Institutes of Health |
| contact email | myriam-gorospe@nih.gov |
| lab head | |
| Krystyna Mazan-Mamczarz | |
| contact affiliation | NIA/NIH |
| contact email | mamczarzkr@nih.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098471/ |
