PXD065902 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Endothelins positively influences the proliferation vs. migration balance of IDH1-mutant glioma cells and promotes proneural to mesenchymal transition |
| Description | Adult diffuse gliomas are the deadliest brain tumours including IDH-wildtype glioblastomas of worst prognosis and diffuse low grade IDH-mutant astrocytomas and oligodendrogliomas. These glial tumours display distinct tumoral cell population defeating current therapies. Our group has previously unveiled the role of NOTCH signalling in glioblastoma cell plasticity and in the conversion of oligodendrocytic-like to astrocytic-like tumoral cells in IDH-mutant low-grade gliomas which escalate inevitably to higher grade malignant gliomas. To gain insight into signalling pathways regulating glioma cell plasticity and malignancy, we focused our work on endothelin signalling including endothelin peptide ligands (ET-1, ET-2, ET-3) binding to G-protein coupled endothelin receptors A and B (EDNRA, EDNRB). Here, using glioma patient samples and glioma patient-derived cell lines, we showed that endothelin reduces glioma cell proliferation while increasing migration initiating a proneural to mesenchymal transition. Mechanistically, EDNRB activation led to IP3-dependent calcium mobilization, apamin-sensitive KCNN2/KCNN3 potassium currents and phosphorylation of ERK1/2 and STAT3 in glioma cells. Finally, we studied endothelin receptor regulation by tumoral microenvironment stimuli highlighting a role for EDNRA induced by NOTCH1 and hypoxia in perivascular hypoxic area in glioblastoma. Altogether, this study demonstrates endothelin signalling as a key player in mesenchymal transformation of diffuse IDH-mutant gliomas and glioblastomas. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-11 |
| AnnouncementXML | Submission_2026-03-11_06:52:02.584.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD065902 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Functional Proteomics Platform FPP |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-08 06:26:32 | ID requested | |
| ⏵ 1 | 2026-03-11 06:52:03 | announced | |
Publication List
Keyword List
| submitter keyword: Human, LGG275 a IDH-mutant low grade astrocytomas patient-derived cell line, TMT-MS/MS |
Contact List
| Jean-Philippe HUGNOT |
|---|
| contact affiliation | Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France |
| contact email | jean-philippe.hugnot@umontpellier.fr |
| lab head | |
| Functional Proteomics Platform FPP |
|---|
| contact affiliation | FPP CNRS |
| contact email | contact@fpp.cnrs.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065902 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065902
- Label: PRIDE project
- Name: Endothelins positively influences the proliferation vs. migration balance of IDH1-mutant glioma cells and promotes proneural to mesenchymal transition
