PXD065863

PXD065863 is an original dataset announced via ProteomeXchange.

Title	Classical virulence factors do not define Cryptococcus pathogenic diversity
Description	Pathogenic determinants in the Cryptococcus genus have been extensively studied, often using standard laboratory isolates. Here, we analyzed the virulence of ten Cryptococcus isolates from diverse sources, species, and genotypes. These isolates exhibited marked differences in their ability to colonize and kill the invertebrate host Galleria mellonella, as well as in their interactions with hemocytes. Capsule formation also varied widely among isolates, with no clear correlation between virulence in G. mellonella and source of isolation, species, fungal burden, capsule size, or interaction with larval hemocytes. To further investigate the basis of this pathogenic diversity, we selected two hypervirulent isolates (C. deuterogattii and C. neoformans) and two hypovirulent isolates (C. gattii and C. neoformans) for in-depth analysis. Differences in the induction of antimicrobial peptides during infection did not account for the observed variation in virulence. Genomic analysis of capsule- related genes, scanning electron microscopy of capsule morphology in vitro and in vivo, and nuclear magnetic resonance of the major capsular polysaccharide all revealed high variability among isolates, but none of these features correlated with virulence. Proteomic profiling of cellular extracts showed that virulent strains were enriched in proteins associated with oxidative processes. Supplementation with an antioxidant during infection increased the virulence of hypovirulent isolates in G. mellonella. These results reveal a high pathogenic diversity in Cryptococcus that goes beyond its classical virulence factors.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:33:40.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlon D M Santos
SpeciesList	scientific name: Cryptococcus neoformans var. neoformans JEC21; NCBI TaxID: NEWT:214684; scientific name: Cryptococcus gattii VGII R265; NCBI TaxID: NEWT:294750;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 12:28:46	ID requested
⏵ 1	2025-10-19 16:33:41	announced

10.1016/j.tcsw.2025.100153;

Sena BAG, Santos MDM, Souza CM, Camillo-Andrade AC, de Oliveira HC, Dos Reis FCG, Castelli RF, Kuczera D, Antoniolli HRM, Santos HGG, Staats CC, Sassaki GL, Carvalho PC, Rodrigues ML, extends beyond classical virulence factors. Cell Surf, 14():100153(2025) [pubmed]

submitter keyword: diversity,Virulence, pathogenic, Cryptococcus

Marcio L. Rodrigues
contact affiliation	Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
contact email	marcio.rodrigues@fiocruz.br
lab head
Marlon D M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065863
     1. Label: PRIDE project
     2. Name: Classical virulence factors do not define Cryptococcus pathogenic diversity