PXD065829

PXD065829 is an original dataset announced via ProteomeXchange.

Title	Proteomic sequencing on the renal cortices of wild-type and TRIM65 knockout diabetic kidney disease mice
Description	The proteomic sequencing on the renal cortices of wild-type (WT) and TRIM65 knockout (KO) diabetic kidney disease (DKD) mice, focusing on the renal tubules, which are the predominant structures within the renal cortex. DKD mouse model: The streptozotocin (STZ)-induced diabetes model combined with unilateral nephrectomy was used to replicate the mice DKD model. Unilateral nephrectomy was under isoflurane anesthesia. Before established DKD mice model we established a baseline. In short, the mice were fed a high-fat diet for 2 weeks and then underwent unilateral nephrectomy. The high-fat diet was continued for another 2 weeks, and STZ (40 mg/kg/5 days) was intraperitoneally injected to the mice. Tail vein blood glucose was measured after 72 h. The establishment of the diabetes model was considered successful if fasting blood glucose ≥ 11.1 mmol/L or random blood glucose ≥ 16.7 mmol/L. Seven days after the STZ injection, blood glucose was measured again, and mice with stable blood glucose within 7 days were categorized as successfully established diabetic mice models. At 24 weeks after the induction of diabetes, mice were sacrificed, and their kidneys were harvested. Mice fed with normal chow without any manipulation were used as the control group.
HostingRepository	iProX
AnnounceDate	2025-07-06
AnnouncementXML	Submission_2025-07-06_22:42:27.751.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Liang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-06 22:42:06	ID requested
⏵ 1	2025-07-06 22:42:28	announced

Dataset with its publication pending

submitter keyword: TRIM65, diabetic kidney disease, Proteomic sequencing

Zhen Liang
contact affiliation	Peking University Shenzhen Hospital
contact email	liangzhen@pkuszh.com
lab head
Zhen Liang
contact affiliation	Peking University Shenzhen Hospital
contact email	liangzhen@pkuszh.com
dataset submitter

iProX dataset URI