PXD065821

PXD065821 is an original dataset announced via ProteomeXchange.

Title	Global profiling of protein myristoylation in the second-generation merozoites of Eimeria tenella
Description	Avian coccidiosis, which is characterized by intestinal damage in the host, is caused by the intracellular protozoan of Eimeria species, particularly Eimeria tenella. During its developmental cycle, various transitional stages are undergone by E. tenella, including oocysts, sporocysts, merozoites, and gametocytes. These transitions need both transcriptional and translational regulation, as well as post-translational protein modification. Myristoylation is a is a post-translational modification (PTM) of proteins that acts as a regulatory mechanism in signal transduction, cellular localization, and even oncogenesis. Previous studies showed that protein myristoylation is crucial for Plasmodium falciparum, Leishmania donovani, Trypanosoma brucei and Toxoplasma gondii. However, little information has been found on enzymes related to myristoylation or the role of protein myristoylation in E. tenella. Therefore, the myristoylation of the second-generation E. tenella merozoite was explored. In this study, we investigated the myristoylated proteome of E. tenella using chemoproteomic methods. We identified 104 proteins that involved in host cell invasion, motility, parasite morphology as well as ribosome components. In addition, proteasome component, electron transport chain and carbohydrate metabolism were identified. This study revealed the significant impact of myristoylation on E. tenella biology and pathogenicity. In brief, this study lays the foundation for future in-depth analysis and functional validation of myristoylated proteome in the life cycle of the avian parasite.
HostingRepository	iProX
AnnounceDate	2025-07-05
AnnouncementXML	Submission_2025-07-06_21:02:28.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zigang Qu
SpeciesList	scientific name: Eimeria tenella; NCBI TaxID: 5802;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-06 21:02:15	ID requested
⏵ 1	2025-07-06 21:02:29	announced

Dataset with its publication pending

submitter keyword: Myristoylation, E. tenella, Biological process, The second-generation merozoites

Zigang Qu
contact affiliation	Lanzhou veterinary research institute, Chinese Academy of Agricultural Sciences
contact email	quzigang@caas.cn
lab head
Zigang Qu
contact affiliation	Lanzhou veterinary research institute, Chinese Academy of Agricultural Sciences
contact email	quzigang@caas.cn
dataset submitter

iProX dataset URI