PXD065818

PXD065818 is an original dataset announced via ProteomeXchange.

Title	Precise and in vivo-compatible spatial proteomics via biolumines-cence-triggered photocatalytic proximity labeling
Description	Protein function is closely tied to its localization and interactions, which can be mapped using proximity labeling (PL). Traditional PL methods, such as peroxidases and biotin ligases, suffer from toxicity or high background. While visible light-triggered photocatalytic labeling offers great potential, it is limited by light-induced background and restricted in vivo applications. Here, we present BRET-ID, an in vivo-compatible PL technology for precise mapping of membraneless organelles and transient protein-protein interactions with sub-minute temporal resolution. BRET-ID combines a genet-ically encoded photocatalyst and NanoLuc luciferase, locally generating blue light to activate the photocatalyst via biolu-minescence resonance energy transfer (BRET). This activation produces singlet oxygen, which oxidizes nearby proteins for analysis with a streamlined chemoproteomic workflow. BRET-ID enables precise mapping of ER membrane proteins without the need for spatial reference-based filtering. Leveraging its high temporal resolution, BRET-ID provides 1-minute snapshots of dynamic GPCR interactions during ligand-induced endocytosis. Additionally, BRET-ID identifies G3BP1-interacting proteins in arsenite-stressed cells and tumor xenografts, uncovering novel stress granule components, including the mTORC2 subunit RICTOR. BRET-ID serves as a powerful genetically encoded tool for studying protein local-ization and molecular interactions in living organisms.
HostingRepository	iProX
AnnounceDate	2025-07-04
AnnouncementXML	Submission_2025-07-06_19:16:04.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuege Sun
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-06 19:15:29	ID requested
⏵ 1	2025-07-06 19:16:04	announced

Dataset with its publication pending

submitter keyword: Proximity labeling, BRET, spatial proteomics, protein-protein interactions, protein engineering

Wei Qin
contact affiliation	Tsinghua university
contact email	weiqin@tsinghua.edu.cn
lab head
Xuege Sun
contact affiliation	Tsinghua university
contact email	zhiyewenku@163.com
dataset submitter

iProX dataset URI