⮝ Full datasets listing
PXD065772
PXD065772 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of MetaAggregates in amyotrophic lateral sclerosis brains |
| Description | RNA-binding proteins (RBPs) are key regulators of translation and have been implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS). Among candidate pathological entities are heterogeneous co-aggregates, or “MetaAggregates,†composed of amyloids, RBPs, and RNA G-quadruplexes (rG4s). To explore their molecular constituents, we developed a proteomic approach using a psoralen-conjugated RBP to crosslink with a biotinylated rG4, enabling isolation of MetaAggregates from ALS brain extracts. Single-cell RNA-seq of ALS iPS-derived neurons identified ELAVL4 as a cytoplasmic RNA-binding protein and revealed enrichment of an IGFBP2-derived rG4 structure in ALS-specific neurons. Label-free LC-MS/MS and amyloidogenicity-based principal component analysis revealed 79 candidate proteins with roles in RNA processing, metabolism, trafficking, and stress responses. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-07-04 |
| AnnouncementXML | Submission_2026-07-04_08:00:34.609.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Takumi Nishiuchi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-03 21:33:58 | ID requested | |
| ⏵ 1 | 2026-07-04 08:00:35 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ALS, Brain, DDA |
Contact List
| Kazuma Murakami | |
|---|---|
| lab head | |
| Takumi Nishiuchi | |
| contact affiliation | Kanazawa University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003914/ |
