PXD065754

PXD065754 is an original dataset announced via ProteomeXchange.

Title	Determination of animal species origin of blood in artworks by LC-MS/MS
Description	For art historians, understanding the animal origin of blood used in artworks is crucial for interpreting materials, techniques, and historical practices. It provides insight into the cultural, and religious significance of the work and allows for a more nuanced interpretation of its symbolic meaning. In this work, sets of peptides of blood proteins, more specifically their amino acid sequences, that are responsible for the differences between seven animal species (cat, cow, dog, goose, hen, human, and pig) were identified by LC-MS/MS. Protein materials used for the preparation of six model blood coatings were confirmed and the animal origin of the used blood was found. Finally, the blood origin in four samples taken from a set of Japanese and Chinese lacquer artworks from 18th and 19th century was also determined.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-22_03:26:09.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiri Santrucek
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Felis catus (Cat) (Felis silvestris catus); NCBI TaxID: 9685; scientific name: Anser cygnoides; NCBI TaxID: 8845; scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; monohydroxylated proline
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 09:49:15	ID requested
⏵ 1	2025-09-22 03:26:10	announced

10.1039/D5AY01156F;

submitter keyword: cultural heritage,Blood coatings, LC-MS/MS

Stepanka Kuckova
contact affiliation	Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague
contact email	Stepanka.Kuckova@vscht.cz
lab head
Jiri Santrucek
contact affiliation	University of Chemistry and Technology Prague
contact email	santrucj@vscht.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD065754

PRIDE project URI

• PRIDE
  1. PXD065754
     1. Label: PRIDE project
     2. Name: Determination of animal species origin of blood in artworks by LC-MS/MS