⮝ Full datasets listing
PXD065747
PXD065747 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Live Imagine and Interactome Analysis of Zika and Chikungunya Viral RNAs Via Dual Action Aptamer Tag |
| Description | Arthropod borne, positive sense RNA viruses encompass many human pathogens posing significant health threats worldwide. Understanding intracellular dynamics and host factor interactions of the viral genome is essential for devising effective antiviral strategies. We engineered dual labeled Zika and Chikungunya viruses incorporating a Mango-II aptamer for RNA pulldown and real time imagine, achieving high resolution imaging of flavivirus RNA in live cells. Tandem imagine with viral replication proteins revealed intracellular dynamics of replication complexes and translating RNA of these viruses. We identified over 1000 high confidence host interactors of viral RNA and corroborated 355 hits from existing data sets. Spliceosome factor SFPQ was identified as a common interactor decreasing RNA production in both viruses when depleted. intracellular colocalization of SFPQ-ZIKV RNA was confirmed through super resolution imagine of infected cells. Thus we demonstrate Mango-II aptamer tagging as an innovative strategy for identifying spatiotemporal dynamics and virus host interactions of viral genomes in positive strand RNA viruses. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-04 |
| AnnouncementXML | Submission_2026-08-04_08:57:53.942.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Zika virus; NCBI TaxID: 64320; scientific name: Chikungunya virus; NCBI TaxID: 37124; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Zika virus MR766; scientific name: Chikungunya virus 181/25; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Exploris 480; Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-03 08:38:39 | ID requested | |
| ⏵ 1 | 2026-08-04 08:57:54 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: zika virus, Chikungunya virus, RNA imaging, Proteomics, Aptamer, SFPQ, DatasetType:Proteomics |
Contact List
| Joyce Jose | |
|---|---|
| contact affiliation | The Pennsylvania State University |
| contact email | jxj321@psu.edu |
| lab head | |
| Amber L. Mosley | |
| contact affiliation | Indiana University School of Medicine |
| contact email | almosley@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098425/ |
