PXD065745

PXD065745 is an original dataset announced via ProteomeXchange.

Title	A protein-centric mass spectrometry approach for species identification within harmful algal blooms
Description	Harmful algal blooms present severe environmental threats, impacting water quality, aquatic ecosystems, and human health. The frequency and intensity of these blooms are rising, largely driven by global warming and changing climatic conditions. There is an urgent need for innovative methods to monitor blue-green algae, also known as cyanobacteria, to enable the implementation of preventative measures. Here, we show that native mass spectrometry is an effective tool for detecting cyanobacteria directly from lake samples, both prior and during bloom formation. Our approach allows for the rapid characterization of cyanobacterial populations within lakes, offering valuable insights into the dynamics of cyanobacterial species associated with harmful algae blooms. Overall, we highlight the exceptional capability of native mass spectrometry in directly detecting and monitoring cyanobacterial blooms, which will support the development of more effective strategies to mitigate this growing environmental challenge.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-28_04:00:22.722.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065745
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aneika Leney
SpeciesList	scientific name: Cyanobacteria; NCBI TaxID: 1117;
ModificationList	N4-methyl-L-asparagine; L-methionine removal; monohydroxylated residue; S-phycocyanobilin-L-cysteine; S-phycoerythrobilin-L-cysteine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 08:13:48	ID requested
⏵ 1	2025-07-28 04:00:23	announced

10.6019/PXD065745;

10.1021/JACS.5C07419;

submitter keyword: native mass spectrometry,Cyanobacteria, bottom-up proteomics, phycobiliproteins, cyanotoxin quantification

Aneika C. Leney
contact affiliation	University of Birmingham
contact email	a.leney@bham.ac.uk
lab head
Aneika Leney
contact affiliation	University of Birmingham
contact email	a.leney@bham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD065745

PRIDE project URI

• PRIDE
  1. PXD065745
     1. Label: PRIDE project
     2. Name: A protein-centric mass spectrometry approach for species identification within harmful algal blooms