⮝ Full datasets listing
PXD065720
PXD065720 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of linear polyubiquitinated substrates via GST-UBAN pulldown enrichment. |
| Description | In this project, we revealed that Sharpin deletion significantly inhibits tumor growth and reprograms the tumor microenvironment. To identify the potential substrates that responsible for the phenotypes, we enriched substrates with linear ubiquitin chains in WT and Sharpin KO EO771 cells and determined substrates with profound decreased level in linear ubiquitination upon Sharpin deletion. For this, cells were lysed with NP-40 lysis buffer in the presence of Protease phosphatase inhibitor cocktail, 1mM DTT and 5mM N-Ethylmaleimide. Purified GST-UBAN bound to glutathione Sepharose 4B beads were incubated with cell lysates at 4 °C overnight. Subsequently, the beads were washed four times with GST binding buffer, and the bound proteins were eluted. The eluted proteins were then subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis using data-independent acquisition (DIA) mode. |
| HostingRepository | iProX |
| AnnounceDate | 2025-07-03 |
| AnnouncementXML | Submission_2026-03-25_19:26:45.218.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenwei Wu |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-03 02:15:30 | ID requested | |
| ⏵ 1 | 2026-03-25 19:26:45 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: GST-UBAN pulldown |
Contact List
| Ruihua Xu | |
|---|---|
| contact affiliation | Department of Medical Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University |
| contact email | xurh@sysucc.org.cn |
| lab head | |
| Wenwei Wu | |
| contact affiliation | Department of Medical Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University |
| contact email | wuww@sysucc.org.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
