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PXD065711
PXD065711 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Disruption of the Golgi-based deacetylase alters the quality of the secretome and neuronal activity |
| Description | The lysine acetylation status of modified proteins depends on acetyltransferases and deacetylases, which often coexist within the same cellular organelle or compartment. An exception is the conventional protein secretion pathway, where acetylation occurs in the lumen of the endoplasmic reticulum (ER) while deacetylation occurs in the lumen of the Golgi apparatus. Here, we provide in vitro and in vivo evidence that C6orf89, a Golgi-based type II membrane protein, has deacetylase activity and behaves as a Zn+2-dependent deacetylase. Furthermore, genetic disruption of C6orf89 in the mouse almost abolished the endogenous Golgi-based deacetylase activity supporting the argument that C6orf89 is a Golgi-based deacetylase (GDAC). Deletion of C6orf89 in the mouse resulted in behavioral defects, expansion of the dendritic spine network, and altered neuronal spontaneous firing. Mechanistically, the mice displayed expansion of the Golgi network with altered N-glycosylation of trafficking glycoproteins. In conclusion, our findings suggest that Golgi deacetylation of transiting N-glycoproteins is required to ensure the quality of the secretome within the conventional protein secretion pathway. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-11 |
| AnnouncementXML | Submission_2026-04-11_11:08:27.728.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Feixuan |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | unknown modification |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-02 21:12:49 | ID requested | |
| ⏵ 1 | 2026-04-11 11:08:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Acetyl-CoA, C6orf89, Deacetylase, Golgi apparatus, N-glycosylation, DatasetType:Proteomics, DatasetType:Other (Glycoproteomics) |
Contact List
| Luigi Puglielli | |
|---|---|
| contact affiliation | University of Wisconsin - Madison |
| contact email | lp1@medicine.wisc.edu |
| lab head | |
| Feixuan | |
| contact affiliation | University of Wisconsin-Madison |
| contact email | fwu76@wisc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098421/ |
