PXD065710

PXD065710 is an original dataset announced via ProteomeXchange.

Title	Quantitation of ten barley-specific amylase/trypsin-inhibitors in the Global EcoSeed panel
Description	Amylase/trypsin-inhibitors (ATIs) are known allergens and triggers of non-celiac wheat sensitivity. Until now, ATIs were only quantitated in wheat species. We developed and validated a targeted stable isotope dilution analysis LC-MS/MS method to quantitate ten barley-specific ATIs, including one monomeric and one dimeric amylase-inhibitor, four chloroform/methanol-soluble types, three subtilisin/chymotrypsin-inhibitors and one amylase/subtilisin-inhibitor. After successful validation in terms of precision, recovery and limits of detection and quantitation, the method was applied to 181 barley accessions from the EcoSeed panel, comprising 113 two-row and 68 six-row barleys of different genetic backgrounds. The overall ATI content was 1.1–5.2 mg/g, corresponding to 0.7–3.6% of the total protein content with no clear distinction between two-row and six-row barleys. This study is the first to provide insights on the ATI content and composition of barley, which can be used to make low-ATI foods for special dietary needs.
HostingRepository	PanoramaPublic
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_19:45:38.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Joestl
SpeciesList	scientific name: Hordeum vulgare; NCBI TaxID: 4513;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-02 20:45:28	ID requested
1	2025-07-02 21:38:46	announced
2	2025-09-10 11:47:48	announced	: Added Publication
⏵ 3	2026-03-05 19:45:39	announced	: Added PubMed ID

Joestl S, Alomari DZ, Alqudah AM, B, ö, rner A, Geisslitz S, Scherf KA, Quantitation of amylase/trypsin-inhibitors in barley using targeted LC-MS/MS. Food Res Int, 218():116910(2025) [pubmed]

submitter keyword: Allergen, LC-MS/MS, Non-celiac gluten sensitivity (NCGS), Non-celiac wheat sensitivity (NCWS), Stable isotope dilution analysis, Amylase/trypsin-inhibitors (ATIs)

Katharina Scherf
contact affiliation	Technical University of Munich, TUM School of Life Sciences, Professorship of Food Biopolymer Systems, Freising, 85354, Germany
contact email	k.scherf.leibniz-lsb@tum.de
lab head
Sarah Joestl
contact affiliation	Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Karlsruhe, 76131, Germany
contact email	sarah.joestl@kit.edu
dataset submitter

Panorama Public dataset URI