PXD065686

PXD065686 is an original dataset announced via ProteomeXchange.

Title	Lipid Metabolism and Contact Site Homologs Are Enriched at the Chloroplast Envelope-Thylakoid Interface
Description	Biogenesis and maintenance of the photosynthetic thylakoid membrane requires transport of lipids from their site of synthesis in the chloroplast envelopes to their destination in the thylakoid. While vesicle trafficking is likely involved, we hypothesized a complementary mechanism involving direct membrane interactions. Using domain homology and proteomic profiling of chloroplast membrane fractions, we identified candidate lipid transport proteins enriched in a distinct, intermediate-density membrane population. This fraction was enriched in lipid metabolic enzymes and proteins homologous to known membrane organization factors. Several candidates, including TVP38 FAMILY PROTEIN (TVPFP), PLASMA MEMBRANE FUSION PROTEIN (PMFP), and LETM1-LIKE, localized to discrete subdomains within chloroplasts. Loss-of-function tvpfp or pmfp mutants exhibited altered chloroplast ultrastructure, including changes in thylakoid-envelope proximity, supporting their roles in maintaining membrane architecture. These findings identify a specialized chloroplast membrane region enriched in lipid-related functions and offer a foundation for elucidating the molecular architecture of these regions.
HostingRepository	PRIDE
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-04-28_21:29:56.693.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD065686
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebecca Roston
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: NEWT:3888;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-02 07:35:32	ID requested
⏵ 1	2026-04-28 21:29:57	announced

10.6019/PXD065686;

submitter keyword: lightThy, heavy inner envelope, light thylakoid, heavyIE,thylakoid, inner envelope

Rebecca L Roston
contact affiliation	University of Nebraska-Lincoln
contact email	rroston@unl.edu
lab head
Rebecca Roston
contact affiliation	University of Nebraska-Lincoln
contact email	rroston@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065686
     1. Label: PRIDE project
     2. Name: Lipid Metabolism and Contact Site Homologs Are Enriched at the Chloroplast Envelope-Thylakoid Interface