PXD065670

PXD065670 is an original dataset announced via ProteomeXchange.

Title	AMPK alters proteasome phosphorylation status and prevents persistent proteasome condensates
Description	Proteasomes undergo dynamic nucleocytoplasmic translocation and phase transitions in response to glucose starvation. AMP-activated protein kinase (AMPK) is important for cytoplasmic proteasome condensate dissolution and proteasome nuclear reentry in budding yeast cells upon glucose refeeding of quiescent cells. This study demonstrates that AMPK interacts with proteasomes, and the AMPK pathway regulates proteasome phosphorylation status and condensate solubility during reversible proteasome condensate formation. AMPK and the PP1 phosphatase dynamically regulate phosphorylation of multiple proteasome subunits. Therefore, the regulation of proteasome phosphorylation by AMPK is likely to be central to proteasome biomolecular condensate formation and dissolution.
HostingRepository	iProX
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-01_20:34:44.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianhui Li
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 20:34:21	ID requested
⏵ 1	2025-07-01 20:34:45	announced

Dataset with its publication pending

submitter keyword: AMPK, Proteasome condensate, Phosphorylation, Glucose starvation, Nucleocytoplasmic translocation, Budding yeast

Mark Hochstrasser
contact affiliation	Yale University
contact email	mark.hochstrasser@yale.edu
lab head
Jianhui Li
contact affiliation	Florida Institute of Technology
contact email	li@fit.edu
dataset submitter

iProX dataset URI